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BIO503 FINAL TERM SOLVED PAPERS
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Of all DNA extraction methods, the organic method (also known as the phenol-chloroform method has been used the longest. That’s because it’s the most effective in extracting large amounts of high molecular weight DNA that was needed for RFLP, which produced the first DNA fingerprints in the 1980s.
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BIO503 FINALTERM PAST PAPERS BY MOAAZ:
This protocol describes a standard method for nucleic acid purification by first extracting phenol: chloroform and then chloroform to remove any remaining phenol. Organic extraction or phenol-chloroform extraction is a liquid-liquid extraction technique in biochemistry and molecular biology for the purification of nucleic acids and protein elimination. Briefly, aqueous samples are mixed with equal volumes of phenol: chloroform mixture.
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The upper aqueous phase is pipetted off and care is taken to avoid pipetting any organic phase or material at the interface. This procedure is often performed multiple times to increase DNA purity. The proteins are divided into the lower organic phase, while the nucleus acids (as well as other impurities such as salts, sugars, etc.) remain at the top aqueous phase