BIO502 FINAL TERM SOLVED PAPERS -

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BIO502 FINAL TERM SOLVED PAPERS

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Genomics:

Of all DNA extraction methods, the organic method (also known as the phenol-chloroform method has been used the longest. That’s because it’s the most effective in extracting large amounts of high molecular weight DNA that was needed for RFLP, which produced the first DNA fingerprints in the 1980s. PRINCIPLE: After mixing, the mixture is centrifuged and the two are separated phases are formed because the phenol:chloroform mixture is immiscible with water. The the aqueous phase is at the top because it is less dense than the organic phase (phenol: chloroform).. ALSO, SEE: Jazz Internet Packages | Daily, Weekly, and Monthly and 3-Day Jazz

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BIO502 FINALTERM PAST PAPERS BY MOAAZ:

This protocol describes a standard method for nucleic acid purification by first extracting phenol: chloroform and then chloroform to remove any remaining phenol. Organic extraction or phenol-chloroform extraction is a liquid-liquid extraction technique in biochemistry and molecular biology for the purification of nucleic acids and protein elimination. Briefly, aqueous samples are mixed with equal volumes of phenol: chloroform mixture.

BIO502 FINAL TERM SOLVED PAPERS:

The upper aqueous phase is pipetted off and care is taken to avoid pipetting any organic phase or material at the interface. This procedure is often performed multiple times to increase DNA purity. The proteins are divided into the lower organic phase, while the nucleus acids (as well as other impurities such as salts, sugars, etc.) remain at the top aqueous phase