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EDU410 FINALTERM PAST PAPERS
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It is a sensitive group test that is used regularly for the detection of all carbohydrates present there in free or combined form. Monosaccharides react rapidly whereas disaccharides and polysaccharides react slowly. Principle: This test makes use of concentrated H2SO4 to catalyze the dehydration of sugars to form hydroxymethyl furfural (from hexoses) or furfural (from pentoses). The furfurals then condense with α-naphthol to give a violet or purple-colored product.
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EDU410 FINAL TERM PAST PAPERS BY MOAAZ:
Different carbohydrates e.g. starch form colored adsorption complexes with iodine due to the adsorption of iodine on the polysaccharide chains. The intensity of the color is dependent on the Practical Manual Biochemistry I (Bio202) 8 length of the unbranched or linear chain accessible for the complex formation.
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Iodine forms complex with starch to give deep blue-black color. Glycogen (highly branched polysaccharide) gives a red color with iodine. Ninhydrin reacts with α-amino acids (-NH2) to give a purple-colored complex. Proteins also have a free amino group on the α carbon that can react with ninhydrin to give a blue-purple product.