BT501 FINAL TERM SOLVED PAPERS

BT501 FINAL TERM SOLVED PAPERS

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Health Biotechnology:

This is the synthesis step where the polymerase carries out the synthesis of the new strand in the 5′ to 3′ direction using a primer and deoxyribonucleoside triphosphates (dNTPs). The average DNA polymerase adds about 1000 bp/minute. Steps 1,2,3 form one cycle and za generally 35-40 such cycles are performed in a typical PCR amplification. ALSO, SEE:

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BT501 FINAL TERM PAST PAPERS:

After completing the cycles, the reaction is maintained for several minutes at 70-74°C allowing full extension of the final extension of the remaining DNA.  The reaction is complete and the resulting amplified nucleic acids are kept at a low-level temperature (~4 °C) until analysis.

BT501 FINAL TERM PAST PAPERS BY MOAAZ:

Vector DNA replication is controlled by the origin of replication and must be recognized by a host factor, especially DNA polymerase, to carry out replication. As a result, there are different types of vector DNA to suit alien cloning DNA in a particular host strain.

BT501 FINAL TERM SOLVED PAPERS:

The vector must replicate in different host strains and the vector needs a special addition of structural features to make it suitable for a particular host strain.  “Why does one vector not replicate in different host strains?”

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