BT501 FINAL TERM SOLVED PAPERS GET PDF PAPERS FILES FROM THE BELOW LINK:
This is the synthesis step where the polymerase carries out the synthesis of the new strand in the 5′ to 3′ direction using a primer and deoxyribonucleoside triphosphates (dNTPs). The average DNA polymerase adds about 1000 bp/minute. Steps 1,2,3 form one cycle and za generally 35-40 such cycles are performed in a typical PCR amplification.
Join VU assignment solution groups and also share with friends. We send solution files, VU handouts, VU past papers, and links to you in these WhatsApp groups. To join WhatsApp groups click the below links.
After completing the cycles, the reaction is maintained for several minutes at 70-74°C allowing full extension of the final extension of the remaining DNA. The reaction is complete and the resulting amplified nucleic acids are kept at a low-level temperature (~4 °C) until analysis.
BT501 FINAL TERM PAST PAPERS BY MOAAZ:
Vector DNA replication is controlled by the origin of replication and must be recognized by a host factor, especially DNA polymerase, to carry out replication. As a result, there are different types of vector DNA to suit alien cloning
DNA in a particular host strain.
BT501 FINAL TERM SOLVED PAPERS:
The vector must replicate in different host strains and the vector needs a special addition of structural features to make it suitable for a particular host strain. “Why does one vector not replicate in different host strains?”