BIO302 FINAL TERM SOLVED PAPERS
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Molecular Biology:
As the reaction proceeds with time, more products are formed. Because the end products inhibit the enzyme, greater inhibition occurs. This is the main problem of colorimetric methods for the determination of this enzyme. On the other hand, in kinetic methods, since enzyme activity is measured during the initial few minutes, the amount of products formed during this short time is negligible to cause any inhibition.
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BIO302 FINALTERM PAST PAPERS BY MOAAZ:
Due to the above problem, it is necessary to standardize any colorimetric method against the standard kinetic method. So this standardization is done against a standard Karmen Unit Assay (Kinetic) and this is extrapolated to different amounts of pyruvate and this has been thoroughly cross-checked.
BIO302 FINAL TERM SOLVED PAPERS:
It is not necessary to plot the standard curve every time an estimate is made. It should be drawn initially when running the first test. Subsequently, a periodic check can be carried out by running only a few pipes, see tubes 1 and 3 of the following table and their outer diameter can be compared with the original curve. Each laboratory should construct its own standard curve according to the procedure table below.
